Are there species limitations for scFAST-seq?
2026-06-23
Answer:
scFAST-seq relies on rRNA blocking, and currently validated rRNA blockers are available for human and mouse.
- Human / Mouse:
Fully supported with optimized rRNA blocker design - Non-human primates (e.g., cynomolgus monkey, rhesus macaque):
Due to high sequence homology with human rRNA, blockers may still be effective, but blocking efficiency may be slightly reduced
- Other species:
Can be processed, but:- rRNA proportion may increase
- Effective mRNA data fraction may decrease
- rRNA proportion may increase
Recommendation:
For non-supported species, consider increasing sequencing depth to compensate for reduced usable data.
文件:
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