Is the single-cell capture throughput sufficient for spatial analysis?

Answer:
SeekSpace typically recovers approximately 10,000–60,000 nuclei per capture area. Most samples yield approximately 10,000–30,000 nuclei.

Capture throughput depends on experimental design, tissue type, and downstream analysis goals. The workflow can be adjusted according to study requirements.

Why partial capture is still sufficient

Spatial transcriptomics does not necessarily require capture of every cell within the tissue.

Spatial localization and cell composition analysis: Cell loss within spatial regions is generally random. As long as representative populations of target cell types are captured, their spatial distribution and functional characteristics can still be analyzed effectively.

Cell-cell interaction analysis: Effective interaction distances between cells are typically within 0–200 μm. The average spacing between captured cells in SeekSpace is approximately 30–150 μm. This range is sufficient for most spatial interaction analyses.