What are the key bioinformatic QC metrics for SeekSpace Spatial Transcriptomics data?

Answer:
The QC report generated by SeekSpaceTools is primarily divided into:

• Joint metrics 
• RNA matrix QC metrics 
• Spatial matrix QC metrics 

Because SeekSpace combines transcriptomic information with spatial localization, both molecular quality and spatial-positioning accuracy must be evaluated together. A dataset with strong RNA metrics but poor spatial localization may still fail to support reliable downstream spatial analysis.

Joint Metrics

These metrics evaluate the integrated performance of transcriptomic and spatial information after tissue-region identification and spatial localization.

Summary Metrics

RNA Matrix QC Metrics

RNA Library QC Metrics

RNA Mapping QC Metrics

Spatial Matrix QC Metrics

These metrics primarily evaluate spatial library integrity, spatial-barcode localization accuracy, spatial signal complexity, and consistency between spatial and transcriptomic information.

Spatial Library Metrics