What are the key bioinformatic QC metrics for the 5' scRNA-seq + Immune Repertoire (TCR/BCR) data?
2026-06-23
Answer:
1. RNA QC Metrics
Follow the same interpretation standards as conventional SeekOne DD 3’ scRNA-seq workflows.
2. TCR Library QC Metrics
The QC metrics for V(D)J can vary dramatically depending on the sample’s intrinsic T/B cell content. For example, if a sample naturally contains few T or B cells—or conversely, if T/B cells are highly enriched—the QC values for V(D)J metrics may show wide fluctuations. Such fluctuations are inherent to the biology of the sample and do not necessarily indicate any issues with the assay, reagents, or the analysis software.
TCR Cell Metrics
TCR Enrichment Metrics
TCR Expression Metrics
TCR Annotation Metrics
3. BCR Library QC Metrics
BCR Cell Metrics
BCR Enrichment Metrics
BCR Expression Metrics
BCR Annotation Metrics
Additional Sequencing QC Metrics for TCR/BCR Libraries
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