What type of DNA methylation does the SeekOne DD single-cell multiome methylation + RNA workflow detect? Can it distinguish 5mC and 5hmC?

2026-06-23

Answer:
The SeekOne DD workflow can detect both 5mC (5-methylcytosine) and 5hmC (5-hydroxymethylcytosine), but it cannot distinguish between them.

Principle overview:

The EM-seq enzymatic conversion workflow (TET2 oxidation + APOBEC3A deamination) converts unmethylated cytosines (C) into thymine (T), while methylated cytosines remain as C signals.
TET2 oxidizes modified cytosines (5mC/5hmC) into protected intermediates.
5hmC is protected through glycosylation and therefore is not converted.
APOBEC3A only deaminates unmethylated cytosines and does not affect methylated or oxidized cytosines.

As a result:

“C” represents methylated cytosines (5mC or 5hmC)
“T” represents unmethylated cytosines

文件：

Previous: Can the SeekOne DD single-cell multiome methylation + RNA workflow use other C to T conversion methods, such as TAPS?

Return List

Next: Does the SeekOne DD single-cell multiome methylation + RNA workflow support immune repertoire enrichment from the same cells?

Answers to popular questions

What are the key bioinformatic QC metrics for SeekSpace Spatial Transcriptomics data?

Does SeekSpace support co-localization of H&E staining and gene expression on the same section?

Which sample types have been validated using SeekSpace?

Is the single-cell capture throughput sufficient for spatial analysis?

How are spatial barcodes integrated with single-cell RNA data for reconstruction, and how is reconstruction accuracy maintained?

What are the recommended OCT embedding procedures and precautions?

Thank You For Your Interest ！

Please fill out this form to view or download the materials you require.

COOKIES

Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy

COOKIES

required

These cookies are necessary for basic functions such as payment. Standard cookies cannot be turned off and do not store any of your information.

analyze

These cookies collect information, such as how many people are using our site or which pages are popular, to help us improve the customer experience. Turning these cookies off will mean we can't collect information to improve your experience.

Feature

These cookies enable the website to provide enhanced functionality and personalization. They may be set by us or by third-party providers whose services we have added to our pages. If you do not allow these cookies, some or all of these services may not function properly.

advertise

These cookies help us understand what you are interested in so that we can show you relevant advertising on other websites. Turning these cookies off will mean we are unable to show you any personalized advertising.