Which thermal cyclers are compatible with the SeekOne DD workflow?
2026-06-23
Answer:
Validated thermal cyclers include:
- Bio-Rad C1000 (discontinued)
- LongGene A300 (deep-well)
Other thermal cyclers may be used if they meet the following requirements:
- Compatible with deep-well plates (≥100 μL volume)
- Provide uniform temperature control
- Equipped with an adjustable heated lid
- Support gradient ramping (~1.5°C/sec) (required for certain workflows such as FAST-seq and SeekSpace spatial transcriptomics)
Allow block cycling to begin before the heated lid reaches the target temperature (to avoid delays)
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