What are the best practices for loading samples onto the SeekOne DD chip?

Answer:
Strict adherence to loading order and technique is critical to ensure stable droplet generation and reproducible results.

Required loading order:

1. Row 1: Cell suspension
   (Prepare the mix first; add cells last) 
2. Row 2: Barcoded beads
   (Equilibrate to room temperature for 30 min; vortex thoroughly before use) 
3. Row 3: Carrier oil 

Key operational guidelines:

• When dispensing reagents, keep the pipette tip ~3 mm below the liquid surface to avoid introducing air bubbles. 
• Barcoded beads are viscous:
  After aspirating to the target volume, wait ~5 seconds before removing the tip to ensure accurate volume transfer. 
• Allow a ~30-second interval between loading each well. 
• For multiple chips:
  Load by row across all chips (all cells → all beads → all oil), rather than completing one chip at a time. 
• During preparation of the cell mixture:
  Add cells last and mix gently but thoroughly before loading. 

After loading all wells:
Ensure no visible bubbles remain at the bottom of the wells.