What happens if the input cell concentration exceeds the recommended range (e.g., >1200 cells/μL)?
2026-06-23
Answer:
Exceeding the recommended cell concentration range may negatively impact both system performance and data quality.
Potential risks:
- Clogging:
High cell density increases the likelihood of aggregates or debris blocking microfluidic channels, potentially leading to failed droplet generation. - Increased doublet rate:
Multiple cells may be encapsulated within the same droplet, complicating downstream analysis. - Reduced effective cell recovery:
At high concentrations, counting accuracy decreases and the actual loaded cell number may deviate from expectations.
Recommendation:
Maintain a target concentration of 700–1200 cells/μL (viable cells).
If the concentration is too high, dilute the sample stepwise using an appropriate resuspension buffer.
文件:
Answers to popular questions
Thank You For Your Interest !
Please fill out this form to view or download the materials you require.
COOKIES
Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy
COOKIES
Our website uses cookies and similar technologies to personalize the advertising shown to you and to help you get the best experience on our website. For more information, see our Privacy & Cookie Policy
These cookies are necessary for basic functions such as payment. Standard cookies cannot be turned off and do not store any of your information.
These cookies collect information, such as how many people are using our site or which pages are popular, to help us improve the customer experience. Turning these cookies off will mean we can't collect information to improve your experience.
These cookies enable the website to provide enhanced functionality and personalization. They may be set by us or by third-party providers whose services we have added to our pages. If you do not allow these cookies, some or all of these services may not function properly.
These cookies help us understand what you are interested in so that we can show you relevant advertising on other websites. Turning these cookies off will mean we are unable to show you any personalized advertising.