What happens if the input cell concentration exceeds the recommended range (e.g., >1200 cells/μL)?

2026-06-23

Answer:
Exceeding the recommended cell concentration range may negatively impact both system performance and data quality.

Potential risks:

  • Clogging:
    High cell density increases the likelihood of aggregates or debris blocking microfluidic channels, potentially leading to failed droplet generation. 
  • Increased doublet rate:
    Multiple cells may be encapsulated within the same droplet, complicating downstream analysis. 
  • Reduced effective cell recovery:
    At high concentrations, counting accuracy decreases and the actual loaded cell number may deviate from expectations.  

Recommendation:

Maintain a target concentration of 700–1200 cells/μL (viable cells).

If the concentration is too high, dilute the sample stepwise using an appropriate resuspension buffer.

 

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