What tissue section thickness is recommended for SeekSpace?
2026-06-23
Answer:
SeekSpace typically recommends frozen tissue sections of approximately 10–20 μm.
Optimal section thickness should be selected based on the diameter of the dominant cell types in the tissue.
Nucleus Diameter Range | Recommended Section Thickness |
3–6 μm | 10 μm |
6–10 μm | 14 μm |
>10 μm | 20 μm |
The goal is to maintain relatively intact nuclear morphology within a single section layer to support accurate single-cell spatial transcriptomic analysis. Final thickness optimization should also consider: tissue type, tissue hardness, cell density, nuclei isolation efficiency and tissue structural integrity.
Important considerations
- Sections that are too thin may reduce RNA signal and nuclei recovery.
- Sections that are too thick may increase barcode diffusion risk, complicate nuclei isolation, increase tissue residue, and elevate spatial background signal.
Blindly increasing section thickness is therefore not recommended.
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