How soon should dissociation and nuclei extraction begin after fresh tissue collection? Can tissue be stored on ice overnight?
2026-06-23
Answer:
Tissue dissociation followed by nuclei extraction should ideally begin within 2 hours after tissue collection, and earlier processing is strongly recommended. Overnight storage on ice is not recommended.
Chromatin accessibility is highly sensitive to ex vivo stress. Although cold conditions slow cellular metabolism, they cannot fully prevent stress-induced chromatin remodeling. Overnight storage may introduce large numbers of artificial open chromatin regions, particularly at stress-response loci, while true biological accessibility signals may be lost.
If immediate processing is not possible, tissues should instead be cut into small pieces, rapidly frozen in liquid nitrogen (within 2 hours after collection), and processed later using a frozen-tissue workflow.
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