Does SeekOne DD Multiome ATAC + RNA support species other than human and mouse?

Answer:
Yes. The workflow is compatible with non-human/non-mouse species, although RNA data quality may be reduced compared with human or mouse samples.

The ATAC portion of the assay is species-independent, because transposase accessibility to open chromatin does not rely on species-specific sequence recognition. The RNA workflow is based on a semi-random priming strategy and can theoretically be applied to any species with an available reference genome.

However, the rRNA blockers included in the kit are currently optimized only for human and mouse samples. For non-model species, rRNA depletion efficiency may decrease, which can lead to:

• Higher proportions of rRNA reads
• Reduced effective mRNA data yield
• Lower genome alignment rates, particularly unique mapping rates

This is not an absolute limitation, but rather an expected performance difference. Many non-model organisms (including rat, macaque, pig, and zebrafish) can still generate usable single-cell multiome datasets.

For non-model species: Expect lower median gene counts compared with human/mouse datasets, while clustering and differential analysis generally remain feasible.