If full-length information is desired, why not directly use third-generation sequencing?

2026-06-23

Answer:
Both approaches have distinct advantagesscFAST-seq is often preferred when cost efficiency, throughput, and non-polyA RNA capture are important.

Dimension

Full-length (NGS-based)

Third-generation sequencing

Cost

Lower

Higher

Throughput

High

Lower

Single-molecule full-length

No (population-level)

Yes

Non-polyA RNA (lncRNA, viral RNA)

Supported (random priming)

Limited (polyA-dependent)

Mutation detection

Supported (requires depth)

Supported (higher error rate)

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