Introduction
Introduction

This kit is designed for isolating high-purity single nuclei from animal tissues. Tissues release intact nuclei through tissue homogenization, lysis of cell membranes and other procedures, while maintaining nuclear membrane stability and chromatin spatial structure. Optimized density gradient centrifugation or column-based techniques further remove cellular debris and other impurities, meeting the quality requirements for nuclei in downstream applications such as Single-Cell Omics and Epigenetics. This product overcomes the limitations of traditional enzymatic digestion methods that rely on sample viability, making it widely applicable to both fresh and fresh-frozen tissues, while remaining compatible with low-input samples ( <10 mg). Featuring a streamlined workflow, this product delivers a standardized solution for complex biological samples.

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Highlights
SeekMate™ Nuclei Isolation Kit
Wide pplicability
Clean background
Intact nuclear morphology with no clumping
SeekMate™ Nuclei Isolation Kit (Column)
Simple and rapid operation
High yield
Intact nuclear morphology with no clumping
Sample Compatibility

Human

Immune System
Lymphoid, Thymus
Respiratory System
Lung, Sinus
Digestive System
Liver, Stomach, Pancreas
Integumentary System
Skin, Eyelid
Reproductive System
Cervix, Endometrium, Mammary gland, Ovary, Placenta
Nervous System
Brain
Urinary System
Kidney, Bladder
Circulatory System
Blood vessel, Heart
Sensory System
Choroid, Olfactory epithelium
Tumors
Glioma, Oral cancer, Nasopharyngeal carcinoma, Throat cancer, Laryngopharyngeal cancer, Melanoma, Stomach cancer, Colorectal cancer, Liver cancer, Pancreatic cancer, Esophageal cancer, Cholangiocarcinoma, Gallbladder cancer, Breast cancer, Renal cancer, Urothelial cancer, Testis cancer, Prostate cancer, Penile cancer, Ovarian cancer, Endometrial cancer, Cervical cancer, Osteosarcoma, Hemangioma, Lung cancer, Lymphoid tumors, Lymphoma, Thyroid cancer, others

Mouse

Nervous System
Brain, Choroid plexus, Spinal cord, Dorsal root ganglia, Trigeminal ganglia, Sympathetic ganglia
Respiratory System
Lung
Urinary System
Kidney
Digestive System
Stomach, Liver, Ileum, Pancreas
Sensory System
Choroid
Circulatory System
Blood vessel, Heart
Reproductive System
Cervix, Mammary gland, Testis
Musculoskeletal System
Lumbar vertebrae, Muscle
Immune System
Lymphoid, Thymus
Integumentary System
Skin, Eyelid
Other
Fat, Embryo

Other Species

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Rat, Monkey, Dog, Pig, Sheep, Yak, Chicken, Lamprey, Scallop, Sea Anemone
Order Information
Product
Product Code
Operation Time
SeekMate™ Nuclei Isolation Kit
YK003-02
~ 50 min
SeekMate™ Nuclei Isolation Kit (Column)
YK004-02
~ 30 min

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