Error: Failed to open file "/path/samplename_SortedByCoordinate.bam.tmp.1020.bam": Too many open files
Release date : Jan 27,2026
- This typically occurs during samtools sort. Check the system's maximum open files per process with ulimit -n. Increase the limit with ulimit -n 8000. If you lack permission, reduce threads and increase memory (e.g., set -@ 1 -m 3G).
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