What are the major risks associated with suboptimal sample quality?
2026-06-18
Answer:
Low cell viability may lead to increased background RNA and reduced separation between true cells and background signals. High aggregation rates can result in doublets or multiplets, which may compromise data accuracy and potentially clog microfluidic channels. Improper cell concentration can introduce counting errors and affect capture efficiency. Low nucleated cell proportion may reduce effective signal, while extreme cell sizes may lead to inaccurate counting or physical blockage of the system.
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